Antigenic Characterization of Excretory / Secretory Antigen of Oesophagostomum Columbianum Using Western Blotting
نویسنده
چکیده
In the present investigation, E/S antigen from O. columbianum was isolated by in vitro culture method and hyper immune serum was raised against this antigen in rabbits. On SDS – PAGE analysis, characterization, the E/S antigen revealed six polypeptides at 29.0, 38.0, 49.0, 71.0, 84.0 and 95.0 kDa. Further, three immuno-reactive polypeptides at 29.0, 38.0 and 49.0 kDa were detected in western blotting. This antigenic characterization study will be useful in developing suitable sero diagnostic in Oesophagostomosis in sheep and goats.
منابع مشابه
Cross antigenicity of immunodominant polypeptides of somatic antigen of Oesophagostomum columbianum with other helminths by western blotting
AIM Oesophagostomum columbianum in small ruminants in India is found as mixed infection commonly in sheep and goat. Haemonchus contortus, an abomasal nematode is found as concurrent infection with it. Eggs of Haemonchus and O. columbianum cannot be easily distinguished. Diagnosis of O. columbianum may only be possible if a non-cross antigenic polypeptide was available for immunodiagnosis. MAT...
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BACKGROUND Cross antigenicity is the major problem in developing a reliable tool for immunodiagnosis and immunoprophylaxis of parasitic diseases. Mixed infection due to different types of gastrointestinal parasites is more common than single species infection under field condition. METHODS The present study was undertaken to detect antigenic cross-reactivity among Haemonchus contortus, Oesoph...
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In the present study, E/S antigen from Bunostomum trigonocephalum was prepared by in vitro culture method and hyper immune serum was raised against this antigen in rabbits. On SDS – PAGE analysis, the E/S antigen revealed six polypeptides at 21.0, 29.0, 47.0, 60.0, 94.0 and 101.0 kDa. Further, three immuno-reactive polypeptides at 29.0, 47.0 and 60.0 kDa were detected in western blotting. This ...
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Excretory/Secretory antigen was prepared by culturing live adult worms of Haemonchus contortus in RPMI 1640 medium at a concentration of 50 worms per mL in a culture-flask at 37 ˚C for 24 hr and the culture supernatant was used as antigen. The E/S antigen was purified by thiol-sepharose affinity chromatography. On western blot analysis, it was demonstrated that thiol-purified antigen showed a s...
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